Detection of new genomic landmarks in the Maltese goat using Rapd PCR

Abstract

Since no information of the Maltese goat genome is available, RAPD technique has been used to identify a number of DNA landmarks. Genome Landmarks have been obtained from the DNA of 66 Maltese goats which were studied with Random Amplification of Polymorphic DNA (RAPD). Eleven (11) reproducible RAPD polymorphic zones were identified. For sequencing, the RAPD zones were cloned into the Puc 18 vector utilising E. coli and then sequenced using both the forward (universal) and reverse primers specific for the Puc 18 vector. After sequencing a total of nine new markers (AF078170, AF078171, AF078172, AF078173, AF078174, AF078175, AF078176, AF078177, AF078178) amounting to approximately 5,500 bp of sequence from the goat genome were identified and their homologies with known nucleic acid and protein databases were described. The new sequences could provide useful anchors for more extensive mapping and sequencing and for genomics assisted breeding of the Maltese goat.