Please use this identifier to cite or link to this item:
https://www.um.edu.mt/library/oar/handle/123456789/22763
Title: | Selective activation of 5-HT2C receptors stimulates GABA-ergic function in the rat substantia nigra pars reticulate : a combined in vivo electrophysiological and neurochemical study |
Authors: | Invernizzi, Roberto William Pierucci, Massimo Calcagno, Eleonora Di Giovanni, Giuseppe Di Matteo, Vincenzo Benigno, Arcangelo Esposito, Ennio |
Keywords: | Substantia nigra Basal ganglia GABAergic neurons Receptor, Serotonin, 5-HT2C Electrophysiology Microdialysis |
Issue Date: | 2007 |
Publisher: | Pergamon Press |
Citation: | Invernizzia, R. W., Pierucci, M., Calcagno, E., Di Giovanni, G., Di Matteo, V., Benignoc, A., & Esposito, E. (2007). Selective activation of 5-HT2C receptors stimulates GABA-ergic function in the rat substantia nigra pars reticulate : a combined in vivo electrophysiological and neurochemical study. Neuroscience, 144(4), 1523-1535. |
Abstract: | In vivo electrophysiology and microdialysis were used to investigate the physiological role of 5-HT2C receptors in the control of substantia nigra pars reticulata (SNr) function. Extracellular single-unit recordings were performed from putative GABA-containing neurons in the SNr of anesthetized rats, and local GABA release was studied by in vivo microdialysis in the SNr of awake freely-moving rats. Systemic administration of the selective 5-HT2C receptor agonist (S)-2-(chloro-5-fluoro-indol-1-yl)-1-methylethylamine 1:1 C4H4O4 (RO 60-0175) caused a dose-dependent excitation of about 30% of the SNr neurons recorded. However, the remaining neurons were either inhibited or unaffected by systemic RO 60-0175, in similar proportion. Local application of RO 60-0175 by microiontophoresis caused excitation in the majority of SNr neurons tested (48%), whereas a group of neurons was inhibited (16%) or unaffected (36%). Both the excitatory and the inhibitory effects of systemic and microiontophoretic RO 60-0175 were completely prevented by pretreatment with SB 243213 [5-methyl-1-({2-[(2-methyl-3-pyridyl)oxy]-5-pyridyl}carbamoyl)-6-trifluoromethylindoline], a selective and potent 5-HT2C receptor antagonist. Consistent with these electrophysiological data, both systemic and intranigral administration of RO 60-0175 and m-chlorophenylpiperazine (mCPP), a non-selective 5-HT2C agonist, markedly increased extracellular GABA levels in the SNr. The stimulatory effect of systemic and local RO 60-0175 on GABA release was completely prevented by systemic administration of SB 243213, whereas local application of SB 243213 into the SNr only partially blocked RO 60-0175-induced GABA release. It is concluded that selective activation of 5-HT2C receptors stimulates GABA-ergic function in the SNr, and the clinical relevance of these data is discussed. |
URI: | https://www.um.edu.mt/library/oar//handle/123456789/22763 |
Appears in Collections: | Scholarly Works - FacM&SPB |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s2.0-S0306452206015211-main.pdf Restricted Access | 1.05 MB | Adobe PDF | View/Open Request a copy |
Items in OAR@UM are protected by copyright, with all rights reserved, unless otherwise indicated.