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https://www.um.edu.mt/library/oar/handle/123456789/105861| Title: | A new diagnostic method for the rapid detection of leishmaniasis using PCR |
| Other Titles: | Trends in pathology in Malta : papers presented at the Third Maltese Medical School Conference 30 November - 2 December 1995 |
| Authors: | Xuereb Anastasil, A. McNerney, R. |
| Keywords: | Amastigotes Leishmaniasis Polymerase chain reaction -- Diagnostic use |
| Issue Date: | 1995 |
| Publisher: | Malta : Department of Pathology, University of Malta |
| Citation: | Xuereb Anastasil, A. & McNerney, R. (1995). A new diagnostic method for the rapid detection of leishmaniasis using PCR. In M. N. Cauchi (Ed.) Trends in pathology in Malta: papers presented at the Third Maltese Medical School Conference 30 November-2 December 1995 (pp. 59-63). Malta : Department of Pathology, University of Malta. |
| Abstract: | A new method has been designed for the early detection of Leishmania donovani from blood using DNA technology. The designed PCR was based on the detection of the Lmet2 repeat sequence which is an imperfect 60-bp repeat sequence and is specific to L. donovani complex. Two PCR primers, Lmet2A and Lmet2B and a short capture probe, Lmet2P are used. To improve PCR specificity, a simplified post-hybridization step has been developed which includes the probe in the PCR and enables the hybridization to be performed automatically as part of the PCR programme. After PCR, the hybrids are detected by capture in microtiter wells and colorimetric visualization. Preliminary results have indicated that this method is diagnostic for Leishmania donovani. |
| URI: | https://www.um.edu.mt/library/oar/handle/123456789/105861 |
| Appears in Collections: | Trends in Pathology in Malta - Papers presented at the Third Maltese Medical School Conference [30 November - 2 December 1995] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| A new diagnostic method for the rapid detection of leishmaniasis using PCR.pdf | 210.54 kB | Adobe PDF | View/Open |
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