Please use this identifier to cite or link to this item:
https://www.um.edu.mt/library/oar/handle/123456789/117921
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Marotta, Diane | - |
dc.contributor.author | Karar, Jayashree | - |
dc.contributor.author | Jenkins, W. Timothy | - |
dc.contributor.author | Kumanova, Monika | - |
dc.contributor.author | Jenkins, Kevin W. | - |
dc.contributor.author | Tobias, John W. | - |
dc.contributor.author | Baldwin, Donald | - |
dc.contributor.author | Hatzigeorgiou, Artemis | - |
dc.contributor.author | Alexiou, Panagiotis | - |
dc.contributor.author | Evans, Sydney M. | - |
dc.contributor.author | Alarcon, Rodolfo | - |
dc.contributor.author | Maity, Amit | - |
dc.contributor.author | Koch, Cameron | - |
dc.contributor.author | Koumenis, Constantinos | - |
dc.date.accessioned | 2024-01-29T13:23:19Z | - |
dc.date.available | 2024-01-29T13:23:19Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Marotta, D., Karar, J., Jenkins, W. T., Kumanova, M., Jenkins, K. W., Tobias, J. W., ... & Koumenis, C. (2011). In vivo profiling of hypoxic gene expression in gliomas using the hypoxia marker EF5 and laser-capture microdissection. Cancer research, 71(3), 779-789. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar/handle/123456789/117921 | - |
dc.description.abstract | Hypoxia is a key determinant of tumor aggressiveness, yet little is known regarding hypoxic global gene regulation in vivo. We used the hypoxia marker EF5 coupled with laser-capture microdissection to isolate RNA from viable hypoxic and normoxic regions of 9L experimental gliomas. Through microarray analysis, we identified several mRNAs (including the HIF targets Vegf, Glut-1, and Hsp27) with increased levels under hypoxia compared with normoxia both in vitro and in vivo. However, we also found striking differences between the global in vitro and in vivo hypoxic mRNA profiles. Intriguingly, the mRNA levels of a substantial number of immunomodulatory and DNA repair proteins including CXCL9, CD3D, and RAD51 were found to be downregulated in hypoxic areas in vivo, consistent with a protumorigenic role of hypoxia in solid tumors. Immunohistochemical staining verified increased HSP27 and decreased RAD51 protein levels in hypoxic versus normoxic tumor regions. Moreover, CD8+ T cells, which are recruited to tumors upon stimulation by CXCL9 and CXCL10, were largely excluded from viable hypoxic areas in vivo. This is the first study to analyze the influence of hypoxia on mRNA levels in vivo and can be readily adapted to obtain a comprehensive picture of hypoxic regulation of gene expression and its influence on biological functions in solid tumors. Cancer Res; 71(3); 779–89. ©2011 AACR. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | American Association for Cancer Research | en_GB |
dc.rights | info:eu-repo/semantics/restrictedAccess | en_GB |
dc.subject | Gene expression -- Research | en_GB |
dc.subject | Gliomas | en_GB |
dc.subject | Non-coding RNA | en_GB |
dc.subject | MicroRNA | en_GB |
dc.title | In vivo profiling of hypoxic gene expression in gliomas using the hypoxia marker EF5 and laser-capture microdissection | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1158/0008-5472.CAN-10-3061 | - |
dc.publication.title | Cancer research | en_GB |
Appears in Collections: | Scholarly Works - FacHScABS |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
In_vivo_profiling_of_hypoxic_gene_expression_in_gliomas_using_the_hypoxia_marker_EF5_and_laser_capture_microdissection.pdf Restricted Access | 1.93 MB | Adobe PDF | View/Open Request a copy |
Items in OAR@UM are protected by copyright, with all rights reserved, unless otherwise indicated.