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https://www.um.edu.mt/library/oar/handle/123456789/123063| Title: | In vivo transcription of ribosomal RNA in relation to the mitotic cycle in Physarum polycephalum |
| Authors: | Hunter, Gary J. Turnock, G. |
| Keywords: | Transcription Ribosomes RNA -- Analysis Cell cycle Physarum polycephalum |
| Issue Date: | 1985 |
| Publisher: | The Company of Biologists Ltd |
| Citation: | Hunter, G. J., & Turnock, G. (1985). In vivo transcription of ribosomal rna in relation to the mitotic cycle in Physarum polycephalum. Journal of cell science, 76(1), 135-143. |
| Abstract: | We have investigated the transcription of ribosomal RNA in plasmodia of Physarum polycephalum by a combination of pulse-labelling with [3H]uridine and RNA; DNA hybridization. The DNA used for the hybridization was a Hindlll restriction fragment (cloned in bacteriophage λ) of Physarum ribosomal DNA that carries a substantial fraction of the rRNA genes, enabling the ribosomal transcripts in the newly synthesized RNA to be measured. We found that ribosomal RNA constituted about 40 % of the pulse-labelled RNA at all times during the synchronous mitotic cycle. |
| URI: | https://www.um.edu.mt/library/oar/handle/123456789/123063 |
| Appears in Collections: | Scholarly Works - FacM&SPB |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| In_vivo_transcription_of_ribosomal_RNA_in_relation_to_the_mitotic_cycle_in_Physarum_polycephalum.pdf | 476.26 kB | Adobe PDF | View/Open |
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