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DC Field | Value | Language |
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dc.contributor.author | Di Giovanni, Giuseppe | - |
dc.contributor.author | Ferraro, Giuseppe | - |
dc.contributor.author | Sardo, Pierangelo | - |
dc.contributor.author | Galati, Salvatore | - |
dc.contributor.author | Esposito, Ennio | - |
dc.contributor.author | Grutta, Vittorio La | - |
dc.date.accessioned | 2017-10-17T15:00:26Z | - |
dc.date.available | 2017-10-17T15:00:26Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Di Giovanni, G., Ferraro, G., Sardo, P., Galati, S., Esposito, E., & La Grutta, V. (2003). Nitric oxide modulates striatal neuronal activity via soluble guanylate cyclase : an in vivo microiontophoretic study in rats. Synapse, 48, 100-107. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar//handle/123456789/22796 | - |
dc.description.abstract | It is now well established that nitric oxide (NO) acts as a neuromodulator in the central nervous system. To assess the role of NO in modulating striatal activity, single-unit recording was combined with iontophoresis to study presumed spiny projection neurons in urethane-anesthetized male rats. Striatal neurons recorded were essentially quiescent and were therefore activated to fire by the iontophoretic administration of glutamate, pulsed in cycles of 30 sec on and 40 sec off. In this study, iontophoresis of 3-morpholinosydnonimine hydrochloride (SIN 1), a nitric oxide donor, produced reproducible, current-dependent inhibition of glutamate-induced excitation in 12 of 15 striatal neurons, reaching its maximal inhibitory effect (76.2 ± 5.6% below baseline) during the application of a 100 nA current. Conversely, microiontophoretic application of N-ω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase, produced clear and reproducible excitation of glutamate evoked firing in 7 of 10 cells (51.4 ± 2.3%, at 100 nA). To evaluate the involvement of cyclic guanosine monophosphate (cGMP) in the electrophysiological effects produced by the NO donor, the effects of methylene blue, an inhibitor of guanylyl cyclase, on the responses of nine neurons to SIN 1 were tested. In six of nine neurons the effect of SIN 1 was significantly reduced during continuous iontophoretic administration (50 nA) of methylene blue. Taken together, these data show that NO modulates the striatal network and that inhibitory control of the output neurons is involved in this effect. These results also suggest that the effects of nitric oxide on striatal neurons are partially mediated via cGMP. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | Infomed | en_GB |
dc.rights | info:eu-repo/semantics/restrictedAccess | en_GB |
dc.subject | Basal ganglia | en_GB |
dc.subject | Electrophysiology | en_GB |
dc.title | Nitric oxide modulates striatal neuronal activity via soluble guanylate cyclase : an in vivo microiontophoretic study in rats | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1002/syn.10193 | - |
dc.publication.title | Synapse | en_GB |
Appears in Collections: | Scholarly Works - FacM&SPB |
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Giovanni_et_al-2003-Synapse.pdf Restricted Access | 103.91 kB | Adobe PDF | View/Open Request a copy |
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