Please use this identifier to cite or link to this item:
https://www.um.edu.mt/library/oar/handle/123456789/70944
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Azzopardi, Ernest A. | - |
dc.contributor.author | Ferguson, Elaine L. | - |
dc.contributor.author | Thomas, David W. | - |
dc.date.accessioned | 2021-03-10T10:57:22Z | - |
dc.date.available | 2021-03-10T10:57:22Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Azzopardi, E. A., Ferguson, E. L., & Thomas, D. W. (2015). Development and validation of an in vitro pharmacokinetic/pharmacodynamic model to test the antibacterial efficacy of antibiotic polymer conjugates. Antimicrobial Agents and Chemotherapy, 59(4), 1837-1843. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar/handle/123456789/70944 | - |
dc.description.abstract | This study describes the use of a novel, two-compartment, static dialysis bag model to study the release, diffusion, and antibacterial activity of a novel, bioresponsive dextrin-colistin polymer conjugate against multidrug resistant (MDR) wild-type Acinetobacter baumannii. In this model, colistin sulfate, at its MIC, produced a rapid and extensive drop in viable bacterial counts (<2 log10 CFU/ml at 4 h); however, a marked recovery was observed thereafter, with regrowth equivalent to that of control by 48 h. In contrast, dextrin-colistin conjugate, at its MIC, suppressed bacterial growth for up to 48 h, with 3 log10 CFU/ml lower bacterial counts after 48 h than those of controls. Doubling the concentration of dextrin-colistin conjugate (to 2 × MIC) led to an initial bacterial killing of 3 log10 CFU/ml at 8 h, with a similar regrowth profile to 1 × MIC treatment thereafter. The addition of colistin sulfate (1 × MIC) to dextrin-colistin conjugate (1 × MIC) resulted in undetectable bacterial counts after 4 h, followed by suppressed bacterial growth (3.5 log10 CFU/ml lower than that of control at 48 h). Incubation of dextrin-colistin conjugates with infected wound exudate from a series of burn patients (n=6) revealed an increasing concentration of unmasked colistin in the outer compartment (OC) over time (up to 86.3% of the initial dose at 48 h), confirming that colistin would be liberated from the conjugate by endogenous α-amylase within the wound environment. These studies confirm the utility of this model system to simulate the pharmacokinetics of colistin formation in humans administered dextrin-colistin conjugates and further supports the development of antibiotic polymer conjugates in the treatment of MDR infections. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | American Society for Microbiology | en_GB |
dc.rights | info:eu-repo/semantics/restrictedAccess | en_GB |
dc.subject | Colistin -- Therapeutic use | en_GB |
dc.subject | Antibacterial agents | en_GB |
dc.subject | Polymers -- Therapeutic use | en_GB |
dc.subject | Multidrug resistance | en_GB |
dc.subject | Gram-negative bacteria | en_GB |
dc.subject | Nanomedicine | en_GB |
dc.title | Development and validation of an in vitro pharmacokinetic/pharmacodynamic model to test the antibacterial efficacy of antibiotic polymer conjugates | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1128/AAC.03708-14 | - |
dc.publication.title | Antimicrobial Agents and Chemotherapy | en_GB |
Appears in Collections: | Scholarly Works - FacM&SAna |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Development_and_validation_of_an_in_vitro_pharmacokinetic_pharmacodynamic_model_to_test_the_antibacterial_efficacy_of_antibiotic_polymer_conjugates_2015.pdf Restricted Access | 805.4 kB | Adobe PDF | View/Open Request a copy |
Items in OAR@UM are protected by copyright, with all rights reserved, unless otherwise indicated.