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Title: | Lateral habenula 5-HT2c receptor function is altered by acute and chronic nicotine exposures |
Authors: | Bombardi, Cristiano Delicata, Francis Tagliavia, Claudio Grandis, Annamaria Pierucci, Massimo Marino Gammazza, Antonella Casarrubea, Maurizio De Deurwaerdère, Philippe Di Giovanni, Giuseppe |
Keywords: | Serotonin -- Agonists Serotonin -- Receptors Habenula Nicotine addiction -- Treatment Smoking cessation |
Issue Date: | 2021 |
Publisher: | MDPI |
Citation: | Bombardi, C., Delicata, F., Tagliavia, C., Grandis, A., Pierucci, M., Marino Gammazza, A.,...Di Giovanni, G. (2021). Lateral habenula 5-HT2C receptor function is altered by acute and chronic nicotine exposures. International Journal of Molecular Sciences, 22(9), 4775. |
Abstract: | Serotonin (5-HT) is important in some nicotine actions in the CNS. Among all the 5-HT receptors (5-HTRs), the 5-HT2CR has emerged as a promising drug target for smoking cessation. The 5-HT2CRs within the lateral habenula (LHb) may be crucial for nicotine addiction. Here we showed that after acute nicotine tartrate (2 mg/kg, i.p.) exposure, the 5-HT2CR agonist Ro 60-0175 (5–640 µg/kg, i.v.) increased the electrical activity of 42% of the LHb recorded neurons in vivo in rats. Conversely, after chronic nicotine treatment (6 mg/kg/day, i.p., for 14 days), Ro 60-0175 was incapable of affecting the LHb neuronal discharge. Moreover, acute nicotine exposure increased the 5-HT2CR-immunoreactive (IR) area while decreasing the number of 5-HT2CR-IR neurons in the LHb. On the other hand, chronic nicotine increased both the 5-HT2CR-IR area and 5-HT2CR-IR LHb neurons in the LHb. Western blot analysis confirmed these findings and further revealed an increase of 5-HT2CR expression in the medial prefrontal cortex after chronic nicotine exposure not detected by the immunohistochemistry. Altogether, these data show that acute and chronic nicotine exposure differentially affect the central 5-HT2CR function mainly in the LHb and this may be relevant in nicotine addiction and its treatment. |
URI: | https://www.um.edu.mt/library/oar/handle/123456789/86475 |
Appears in Collections: | Scholarly Works - FacM&SPB |
Files in This Item:
File | Description | Size | Format | |
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Lateral_habenula_5_HT2C_receptor_function_is_altered_by_acute_and_chronic_nicotine_exposures_2021.pdf | 4.92 MB | Adobe PDF | View/Open |
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