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https://www.um.edu.mt/library/oar/handle/123456789/93425| Title: | Comparison of DNA extraction methods for samples from old blood collections [poster] |
| Authors: | Samut Tagliaferro, Sarah Zejnelagic, Azra Farrugia, Rosienne Bezzina Wettinger, Stephanie |
| Keywords: | DNA fingerprinting Biobanks Blood -- Collection and preservation DNA fingerprinting -- Technique Polymerase chain reaction |
| Issue Date: | 2020 |
| Publisher: | Springer Nature Limited |
| Citation: | Samut Tagliaferro, S., Zejnelagic, A., Farrugia, R., & Bezzina Wettinger, S. (2021). Comparison of DNA extraction methods for samples from old blood collections. European Journal of Human Genetics, 28, 958. |
| Abstract: | INTRODUCTION: Limited information is available on which
methods are suitable to extract DNA from 20-year-old
blood samples and samples kept in non-ideal storage conditions. The aims of this study were to assess if DNA can be
extracted from whole blood collected and stored at -20 °C
for 7-20 years, and to identify which commercial DNA
extraction kit is most suitable for this purpose, yielding
good quality DNA. MATERIALS AND METHODS: Nine whole blood samples collected in 1999, 2001 and 2012, all having low (between 100 μL to 200 μL) blood volumes, were used in this study. DNA extraction was performed using three different DNA extraction kits, according to their respective instructions. [excerpt] |
| URI: | https://www.um.edu.mt/library/oar/handle/123456789/93425 |
| Appears in Collections: | Scholarly Works - FacHScABS |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Comparison of DNA extraction methods for samples from old blood collections.pdf Restricted Access | 138.77 kB | Adobe PDF | View/Open Request a copy |
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