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Title: | Comparative proteomic analysis of two stress-management strategies in pancreatic cancer |
Authors: | Baron, Byron Fujioka, Tsuyoshi Kitagawa, Takao Maehara, Shin-Ichiro Maehara, Youshihiko Nakamura, Kazuyuki Kuramitsu, Yasuhiro |
Keywords: | Pancreas -- Cancer -- Treatment Proteomics Proteins -- Analysis |
Issue Date: | 2015 |
Publisher: | International Institute of Anticancer Research |
Citation: | Baron, B., Fujioka, T., Kitagawa, T., Maehara, S. I., Maehara, Y., Nakamura, K., & Kuramitsu, Y. (2015). Comparative proteomic analysis of two stress-management strategies in pancreatic cancer. Cancer Genomics & Proteomics, 12(2), 83-87. |
Abstract: | Background: It is known that cancers adopt different strategies to cope with stress and overcome adverse micro-environmental conditions. Such strategies are also applicable to chemo-therapeutic treatment, which could subsequently result in chemo-resistance. Materials and Methods: In order to investigate known stress-evasion strategies observed in pancreatic cancer, the stress-resistant KLM1-derived cell lines KLM1-R (Gemcitabine (GEM)- induced stress) and KLM1-S (growth factor restrictioninduced stress) were employed. Comparative proteomics were employed between for the two cell lines that were also compared against the parent cell line KLM1. Results: Proteomic analysis revealed changes in the expression levels of 6 proteins, namely: transitional endoplasmic reticulum ATPase, lamin A/C, PDZ and LIM protein 1, calmodulin, heat shock protein 60 and alpha enolase. Resistance to GEM of KLM1-R and KLM1-S was found to be comparable, with KLM1-S cells exhibiting close to 1.5-fold higher halfmaximal inhibitory concentration (IC50) compared to KLM1- R cells. Conclusion: These results suggest that KLM1-R can be used as a model of directly-acquired chemoresistance (responding directly to evade GEM treatment), while KLM1- S is a good model of indirectly-acquired chemoresistance (formed in response to having to survive with less availability of growth factors), additionally gaining a selective advantage upon GEM treatment. |
URI: | https://www.um.edu.mt/library/oar/handle/123456789/98487 |
ISSN: | 1109-6535 |
Appears in Collections: | Scholarly Works - CenMMB |
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