Please use this identifier to cite or link to this item:
https://www.um.edu.mt/library/oar/handle/123456789/98584
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tokuda, Kazuhiro | - |
dc.contributor.author | Kuramitsu, Yasuhiro | - |
dc.contributor.author | Baron, Byron | - |
dc.contributor.author | Kitagawa, Takao | - |
dc.contributor.author | Tokuda, Nobuko | - |
dc.contributor.author | Kobayashi, Daiki | - |
dc.contributor.author | Nagayama, Megumi | - |
dc.contributor.author | Araki, Norie | - |
dc.contributor.author | Sonoda, Koh-Hei | - |
dc.contributor.author | Nakamura, Kazuyuki | - |
dc.date.accessioned | 2022-06-30T10:34:56Z | - |
dc.date.available | 2022-06-30T10:34:56Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Tokuda, K., Kuramitsu, Y., Byron, B., Kitagawa, T., Tokuda, N., Kobayashi, D., ... & Nakamura, K. (2015). Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina. Biochemical and Biophysical Research Communications, 463(4), 593-599. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar/handle/123456789/98584 | - |
dc.description.abstract | Glutamate has been shown to induce neural progenitor cells in the adult vertebrate retina. However, protein dynamics during progenitor cell induction by glutamate are not fully understood. To identify specific proteins involved in the process, we employed two-dimensional electrophoresis-based proteomics on glutamate untreated and treated retinal ex vivo sections. Rat retinal tissues were incubated with 1 mM glutamate for 1 h, followed by incubation in glutamate-free media for a total of 24 h. Consistent with prior reports, it was found that mitotic cells appeared in the outer nuclear layer without any histological damage. Immunohistological evaluations and immunoblotting confirmed the emergence of neuronal progenitor cells in the mature retina treated with glutamate. Proteomic analysis revealed the up-regulation of dihydropyrimidinase-related protein 3 (DRP-3), DRP-2 and stress-induced-phosphoprotein 1 (STIP1) during neural progenitor cell induction by glutamate. Moreover, mRNA expression of DRP-3, especially, its long isoform, robustly increased in the treated retina compared to that in the untreated retina. These results may indicate that glutamate induces neural progenitor cells in the mature rat retina by up-regulating the proteins which mediate cell mitosis and neurite growth. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier Inc. | en_GB |
dc.rights | info:eu-repo/semantics/restrictedAccess | en_GB |
dc.subject | Cell organelles -- Formation | en_GB |
dc.subject | Neural stem cells -- Regeneration | en_GB |
dc.subject | Nervous system | en_GB |
dc.subject | Mass spectrometry | en_GB |
dc.subject | Molecular biology | en_GB |
dc.title | Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1016/j.bbrc.2015.05.102 | - |
dc.publication.title | Biochemical and Biophysical Research Communications | en_GB |
Appears in Collections: | Scholarly Works - CenMMB |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Up-regulation_of_DRP-3_long_isoform_during_the_induction_of_neural_progenitor_cells_by_glutamate_treatment_in_the_ex_vivo_rat_retina(2015).pdf Restricted Access | 1.48 MB | Adobe PDF | View/Open Request a copy |
Items in OAR@UM are protected by copyright, with all rights reserved, unless otherwise indicated.