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DC Field | Value | Language |
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dc.contributor.author | Remme, Carol Ann | - |
dc.contributor.author | Verkerk, Arie O. | - |
dc.contributor.author | Hoogaars, W. M. H. | - |
dc.contributor.author | Aanhaanen, W. T. J. | - |
dc.contributor.author | Scicluna, Brendon P. | - |
dc.contributor.author | Annink, C. | - |
dc.contributor.author | Hoff, Maurice J. B. van den | - |
dc.contributor.author | Wilde, Arthur A. M. | - |
dc.contributor.author | Veen, T. A. B. van | - |
dc.contributor.author | Veldkamp, Marieke W. | - |
dc.contributor.author | Bakker, Jacques M. T. de | - |
dc.contributor.author | Christoffels, V. M. | - |
dc.contributor.author | Bezzina, Connie R. | - |
dc.date.accessioned | 2022-06-30T11:11:36Z | - |
dc.date.available | 2022-06-30T11:11:36Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Remme, C. A., Verkerk, A. O., Hoogaars, W. M. H., Aanhaanen, W. T. J., Scicluna, B. P., Annink, C., ... & Bezzina, C. R. (2009). The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardium. Basic Research in Cardiology, 104(5), 511-522. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar/handle/123456789/98603 | - |
dc.description.abstract | Cardiac sodium channels are responsible for conduction in the normal and diseased heart. We aimed to investigate regional and transmural distribution of sodium channel expression and function in the myocardium. Sodium channel Scn5a mRNA and Nav1.5 protein distribution was investigated in adult and embryonic mouse heart through immunohistochemistry and in situ hybridization. Functional sodium channel availability in subepicardial and subendocardial myocytes was assessed using patch-clamp technique. Adult and embryonic (ED14.5) mouse heart sections showed low expression of Nav1.5 in the HCN4-positive sinoatrial and atrioventricular nodes. In contrast, high expression levels of Nav1.5 were observed in the HCN4-positive and Cx43-negative AV or His bundle, bundle branches and Purkinje fibers. In both ventricles, a transmural gradient was observed, with a low Nav1.5 labeling intensity in the subepicardium as compared to the subendocardium. Similar Scn5a mRNA expression patterns were observed on in situ hybridization of embryonic and adult tissue. Maximal action potential upstroke velocity was significantly lower in subepicardial myocytes (mean ± SEM 309 ± 32 V/s; n = 14) compared to subendocardial myocytes (394 ± 32 V/s; n = 11; P < 0.05), indicating decreased sodium channel availability in subepicardium compared to subendocardium. Scn5a and Nav1.5 show heterogeneous distribution patterns within the cardiac conduction system and across the ventricular wall. This differential distribution of the cardiac sodium channel may have profound consequences for conduction disease phenotypes and arrhythmogenesis in the setting of sodium channel disease. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | Springer Medizin | en_GB |
dc.rights | info:eu-repo/semantics/restrictedAccess | en_GB |
dc.subject | Sodium channels | en_GB |
dc.subject | Heart conduction system | en_GB |
dc.subject | Immunohistochemistry | en_GB |
dc.subject | Patch-clamp techniques (Electrophysiology) | en_GB |
dc.title | The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardium | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1007/s00395-009-0012-8 | - |
dc.publication.title | Basic Research in Cardiology | en_GB |
Appears in Collections: | Scholarly Works - FacHScABS |
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