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Title: | Proteomic differential display analysis shows up-regulation of 14-3-3 sigma protein in human scirrhous-type gastric carcinoma cells |
Authors: | Kuramitsu, Yasuhiro Baron, Byron Yoshino, Shigefumi Zhang, Xiulian Tanaka, Toshiyuki Yashiro, Masakazu Hirakawa, Kosei Oka, Masaaki Nakamura, Kazuyuki |
Keywords: | Cancer Gastrointestinal diseases -- Diagnosis Gel electrophoresis Amino acid sequence Medical biochemistry |
Issue Date: | 2010 |
Publisher: | International Institute of Anticancer Research |
Citation: | Kuramitsu, Y., Baron, B., Yoshino, S., Zhang, X., Tanaka, T., Yashiro, M., ... & Nakamura, K. (2010). Proteomic differential display analysis shows up-regulation of 14-3-3 sigma protein in human scirrhous-type gastric carcinoma cells. Anticancer Research, 30(11), 4459-4465. |
Abstract: | This study performed proteomic differential display analysis of human scirrhous-type gastric carcinoma (SGC) cell lines and normal gastric mucosa (NGM) tissues by using twodimensional gel electrophoresis (2-DE) and liquid chromatography–tandem mass spectrometry (LC-MS/MS). The human SGC cell lines were OCUM-1, OCUM-2M, OCUM2MLN, OCUM-2D, OCUM-D3, OCUM-9 and OCUM-12. Among the SGC cell lines and the NGM tissues, 28 protein spots were found whose expression levels were different from the results of 2-DE: 19 protein spots appeared higher, and 9 other protein spots appeared lower in SGCs than in NGM tissues. These spots were analysed by LC-MS/MS analysis and identified by a peptide sequence tag. Identified increased spots included elongation factor 1-beta, 14-3-3 sigma, tropomyosin alpha-4 chain, protein DJ-1, nucleoside diphosphate kinase A, elongation factor Tu and peroxiredoxin-1. Western blot analysis showed increased protein level of 14-3-3 sigma in SGCs. Although OCUM-1 and AGS (gastric cancer) showed up-regulation of 14- 3-3 sigma, MiaPaca-2 (pancreatic cancer), Huh-7 (HCC) and NCI-H2052 (malignant pleural mesothelioma) showed very weak expression of 14-3-3 sigma. The up-regulation of 14-3-3 sigma may play an important role in SGC carcinogenesis and progression and may be used as a diagnostic biomarker of SGC. |
URI: | https://www.um.edu.mt/library/oar/handle/123456789/99195 |
Appears in Collections: | Scholarly Works - CenMMB |
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Proteomic_Differential_Display_Analysis_Shows_Up-regulation_of_14-3-3_Sigma_Protein_in_Human_Scirrhous-type_Gastric_Carcinoma_Cells(2010).pdf Restricted Access | 235.73 kB | Adobe PDF | View/Open Request a copy |
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