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Title: | Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways |
Authors: | Montefort, Stephen Goulding, David Polosa, Riccardo Haskard, Dorian O. Howarth, Peter Hugo Holgate, Stephen T. Carroll, Mary P. Gratziou, Christina G. |
Keywords: | Allergens Asthma Leucocytes Endothelial cells Cell adhesion molecules |
Issue Date: | 1994 |
Publisher: | The American Society for Clinical Investigation |
Citation: | Montefort, S., Gratziou, C., Goulding, D., Polosa, R., Haskard, D. O., Howarth, P. H.,...Carroll, M. P. (1994). Bronchial biopsy evidence for leukocyte infiltration and upregulation of leukocyte-endothelial cell adhesion molecules 6 hours after local allergen challenge of sensitized asthmatic airways. Journal of Clinical Investigation, 93(4), 1411-1421. |
Abstract: | We have examined the mucosal changes occurring in bronchial biopsies from six atopic asthmatics 5-6 h after local endobronchial allergen challenge and compared them with biopsies from saline-challenged segments from the same subjects at the same time point. All the subjects developed localized bronchoconstriction in the allergen-challenged segment and had a decrease in forced expiratory volume in 1 s (FEV1) (P < 0.01) and a decrease in their methacholine provocative concentration of agonist required to reduce FEV1 from baseline by 20% (P < 0.05) 24 h postchallenge. At 6 h we observed an increase in neutrophils (P = 0.03), eosinophils (P = 0.025), mast cells (P = 0.03), and CD3+ lymphocytes (P = 0.025), but not in CD4+ or CD8+ lymphocyte counts. We also detected an increase in endothelial intercellular adhesion molecule type 1 (P < 0.05) and E-selectin (P < 0.005), but not vascular cell adhesion molecule type 1 expression with a correlative increase in submucosal and epithelial LFA+ leucocytes (P < 0.01). Thus, in sensitized asthmatics, local endobronchial allergen instillation leads to an increased inflammatory cell infiltrate of the airway mucosa that involves upregulation of specific adhesion molecules expressed on the microvasculature. |
Description: | This work was supported by a Medical Research Council Programme grant (PG 860 4034). S. Montefort is a Commonwealth Scholar. |
URI: | https://www.um.edu.mt/library/oar//handle/123456789/22876 |
Appears in Collections: | Scholarly Works - FacM&SMed |
Files in This Item:
File | Description | Size | Format | |
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Bronchial biopsy evidence for leukocyte infiltration and upregulation.pdf | 4.63 MB | Adobe PDF | View/Open |
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